The Insulin-Like Growth Factor II (IGF-II) ELISA kit, a quantitative two-step sandwich immunoassay, provides materials for the measurement of IGF-II in serum and other biological fluids. The assay of plasma IGF-II is complicated by the presence of IGF-binding proteins, which may sequester IGF-II in the reaction mixture. Various methods have been devised to separate the IGF-II and IGF-binding proteins prior to assay. Size-exclusion gel chromatography in acid is considered to be optimal, but this procedure is not feasible for routine use. Acidification followed by ethanol or acetone precipitation of the IGF fraction gives results which are similar to acid-chromatography.Direct detection method eliminates the potential altering of sample matrix from size-exclusion chromatography or filtration methods. The IGF-II assay is calibrated to the WHO IGF-II preparation NIBSC Code 96/538 v4.0. Wide dynamic range of 20 - 1239 ng/mL. Sensitive to 1.33 ng/mL. Total 1.5 hr incubation at room temperature. Long shelf life of 24 months.For Research Use Only within the United States. Reference the directional insert on the company's website for more updated specifications as they may have changed.Ansh Labs, located just south of Houston Texas, is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins. Our scientists are experts in immunoassay development and have developed some of the most challenging hormone assays helping to advance research and clinical diagnostics in reproductive function, growth disorders, metabolism, and more.
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