The Intact IGFBP-4 ELISA, a quantitative three-step sandwich immunoassay, provides materials for the measurement of IGFBP-4 in human serum, other biological fluids and cell culture supernatants. The ratio of Total to Intact IGFBP-4 concentration measured in individual subjects over time will help normalizes the IGFBP-4 variability between subjects and also increase the detection rate of increased PAPP-A activity in myocardial infarction subjects. Also useful in research related to cancer studies, hypertension, diabetes, cardiomyopathy, osteoarthritis, aging and longevity studies, and growth hormone deficiency.The monoclonal antibody pair used in the assay detects intact IGFBP-4. The capture antibody binds to the N terminal of the IGFBP-4 molecule whereas detection antibody binds to C terminal. The assay does not cross-react to IGF-I, rat IGF-I, IGFBP-3, IGFBP-3/IGF-1 complex and IGFBP-2 at 1000 ng/ml concentration. The assay also detects bovine and equine IGFBP-4 when tested in serum samples.Analytical measurable range of 7 - 1200 ng/mL. Simple procedure with no sample preparation. Total 2 hr incubation at room temperature. Sensitive to 1.62 ng/mL. Excellent inter- and intra-assay CVs are less than 4.86% and 5.68% respectively.For Research Use Only within the United States. Reference the directional insert on the company's website for more updated specifications as they may have changed.Ansh Labs, located just south of Houston Texas, is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins. Our scientists are experts in immunoassay development and have developed some of the most challenging hormone assays helping to advance research and clinical diagnostics in reproductive function, growth disorders, metabolism, and more.
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